⚬ Services
Code
OSA00071G
$240USD    Buy Now 

ID Tag
Rb672-060908-G

Immunogen
A synthetic peptide from the c-terminal of beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.

Accession

Also known
ACTB, ACTG, ACTK

Target
FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Erba H.P, et al. Nucleic Acids Res. 14:5275-5294(1986).
2. Chang K.-W, et al. Hepatol. Res. 36:33-39(2006).
3. Zahedi R.P, et al. J. Proteome Res. 7:526-534(2008).
4. Procaccio V, et al. Am. J. Hum. Genet. 78:947-960(2006).
5. Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003).

Limitation
For research use only

Related
products
 
Code Product Name
OSA00059W Rabbit antibody to Actin
 

Code
OSA00071G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Actin.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to Actin
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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