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Code
OSV00023W
$240USD    Buy Now 

ID Tag
Rb691-060908-WS

Immunogen
A synthetic peptide from the internal region of human SNF8 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat, mouse and xenopus.

Accession

Also known
EAP30, SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22

Target
FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Schmidt A.E, et al. J. Biol. Chem. 274:21981-21985(1999).
2. von Schwedler U.K, et al. Cell 114:701-713(2003).

Limitation
For research use only

Related
products

Code
OSV00023W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SNF8.

Spcs X-react.
Mouse, rat, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSV00023W
$240USD    Buy Now 


Rabbit antibody to SNF8 WB on rat brain lysate using Rabbit antibody to SNF8 (OSV00023W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

⚬ Products
Rabbit antibody to SNF8
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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