FW (kDa)

Molecule

A₂₈₀ (1mg/ml)

Non-reduced

H

Reduced

L

IgG

1.43

160

55

25

IgM

1.18

900

78

25

Fab

1.53

50

25*

F (ab')₂

1.48

110-120

25*

F (c)

1.41

50

25

F (ab')µ

1.38

65

44

25

F (ab')₂µ

1.38

135

44

25

Fc5µ

1.21

220-250

25

Albumin (Bovine)

0.66

66

-

Albumin (Human)

0.56

667.6

-

Avidin (Human)

1.43

64.3

-

Protein A

0.14

42

-

Protein G

0.95

33 (22.6 actual)

-

*May appear as a doublet on SDS-PAGE.

H: heavy chain
L: light chain

Acid or Base

FW

Molarity

Acetic acid, glacial

60.1

17.4

Formic acid

46.0

23.4

Hydrochloric acid, 38%

36.5

11.6

Hydrofluoric, 55%

20.0

32.1

Nitric acid, 71%

63.0

16.0

Phosphoric acid

98.0

18.3

Sulfuric acid

98.1

18.0

Ammonium hydroxide

35.0

14.8

Ethanolamine, 30%

61.1

16.5

Hydrogen peroxide, 30%

34.0

8.8

ß -Mercaptoethanol

78.1

14.4

Biol.

Act Biological Activity

CS

Cryostat Section

ELISA

Enzyme Linked Immunosorbent Assay

FC

Flow Cytometry

IB

Immunoblotting

IF

Immunofluorescence

IHC

Immunohistochemistry

PS

Paraphin-Embeded Section

RS

Resin Section

WB

Western Blot