Sheep antibody to phospho Y140 & S156 of Cofilin-1 (Cofilin, non-muscle isoform): IgG

$240USD

Code	OSC00053G
ID Tag	Sh102-060908-G
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	500 µg
Immunogen	A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and monkey.
Conjugate	Unconjugated antibody
Accession	COF1_HUMAN COF1_RAT COF1_MOUSE COF1_MONKEY
Also known as	Cofilin, non-muscle isoform
Background	FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm, cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues.
Host	Sheep
Purity	IgG
Clonality	Polyclonal
Isotype	Polyclonal, IgG
Applications	IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.
Specificity	Specific for Cofilin-1.
Spcs X-react.	Mouse, rat, human. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Ogawa K, et al. Nucleic Acids Res. 18:7169-7169(1990). 2. Davidson M.M, et al. Biochem. J. 301:41-47(1994). 3. Nakano K, et al. Exp. Cell Res. 287:219-227(2003). 4. Olsen J.V, et al. Cell 127:635-648(2006). 5. Rikova K, et al. Cell 131:1190-1203(2007).
Limitation	For research use only

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Associated Products

Code	Product Name
OSC00051W	Sheep antibody to phospho Y140 & S156 of Cofilin-1 (Cofilin, non-muscle isoform): whole serum