NEDD8 antibody, Ubiquitin like protein NEDD8 antibody, NEDD-8 antibody

Sheep antibody to n-terminal of NEDD8 (Ubiquitin-like protein Nedd8, Neddylin): IgG

$240USD

Code	OSN00021G
ID Tag	Sh129-070309-G
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	500 µg
Immunogen	A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human, rat, zebrafish, drosophila and xenopus.
Conjugate	Unconjugated antibody
Accession	NEDD8_MOUSE NEDD8_HUMAN NEDD8_RAT NEDD8_DANIO RERIO NEDD8_DROSOPHILA NEDD8_XENOPUS
Also known as	Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8
Background	Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function
Host	Sheep
Purity	IgG
Clonality	Polyclonal
Isotype	Polyclonal, IgG
Applications	IHC (both on frozen and paraffin embedded tissues), WB. Use at a concentration of 10-50 µg/ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for NEDD8.
Spcs X-react.	Mouse, rat, human. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Kumar S, et al. Biochem. Biophys. Res. Commun. 185:1155-1161(1992) 2. Kumar S, et al. Biochem. Biophys. Res. Commun. 195:393-399(1993) 3. Antenos M, et al. J. Biol. Chem. 277:44028-44034(2002) 4. Carrabino S, et al. Gene Expr. Patterns 4:423-431(2004) 5. Hori T, et al. Oncogene 18:6829-6834(1999)
Limitation	For research use only

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MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSN00020W	Sheep antibody to n-terminal of NEDD8 (Ubiquitin-like protein Nedd8, Neddylin): whole serum