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Code
OSC00037W
$240USD    Buy Now 

ID Tag
Sh81-290608-WS

Immunogen
A synthetic peptide from human Cathelicidin antimicrobial peptide conjugated to blue carrier protein has been used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

Target
Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995).
2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995).
3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995).

Limitation
For research use only

Related
products

Code
OSC00037W
$240USD    Buy Now 

Unit size
150 µl

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1:100 to 1: 3000. The optimal dilution should be determined by the end user.

Specificity
Specific for Cathelicidin antimicrobial peptide.

Spcs X-react.
Human, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00037W
$240USD    Buy Now 


Sheep antibody to LL-37 Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: whole serum (OSC00037W), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI.  The antibody selectively recognizes polymorphonuclar cells.
Sheep antibody to LL-37 Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: whole serum (OSC00037W), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.
Sheep antibody to LL-37 WB on human neutrophil lysate using Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): whole serum (OSC00037W) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
Sheep antibody to LL-37 IHC-P on paraffin sections of human large intestine tissue. HIER: Tris-EDTA, pH 9.0 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
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Sheep antibody to LL-37
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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