Sheep antibody to human TRPM4 (Melastatin-4): whole serum

$240USD

Code	OST00033W
ID Tag	Sh43-060108-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide as a part of human transient receptor potential cation channel subfamily M member 4 (TRPM4, melastatin-4) conjugated to immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	TRPM4_HUMAN
Also known as	Transient receptor potential cation channel subfamily M member 4, long transient receptor potential channel 4, hTRPM4, melastatin-4, calcium-activated non-selective cation channel 1, TRPM4, TRPM4B, FLJ20041
Background	FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostaste. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.
Host	Sheep
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for TRPM4.
Spcs X-react.	Human. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Xu X.-Z.S, et al. Proc. Natl. Acad. Sci. U.S.A. 98:10692-10697(2001). 2. Launay P, et al. Cell 109:397-407(2002). 3. Hofmann T, et al. Curr. Biol. 13:1153-1158(2003). 4. Ota T, et al. Nat. Genet. 36:40-45(2004). 5. Earley S, et al. Circ. Res. 95:922-929(2004). 6. Launay P, et al. Science 306:1374-1377(2004). 7. Nilius B, et al. J. Biol. Chem. 280:22899-22906(2005). 8. Zhang Z, et al. J. Biol. Chem. 280:39185-39192(2005). 9. Cheng H, et al. Cell Calcium 41:51-61(2007). 10. Takezawa R, et al. Mol. Pharmacol. 69:1413-1420(2006).
Limitation	For research use only

Sheep antibody to human TRPM4 (Melastatin-4): whole serum

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

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OSR00134W	Rabbit antibody to human TRPM4 (Melastatin-4): whole serum