Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): whole serum

$240USD

Code	OSC00037W
ID Tag	Sh81-290608-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	150 µl
Immunogen	A synthetic peptide as a part of human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39) conjugated to an immunogenic carrier protein has been used as the immunogen.
Conjugate	Unconjugated antibody
Accession	CAMP_HUMAN
Also known as	CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26
Background	Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.
Host	Sheep
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. Use at a dilution of 1:100 to 1: 3000. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for Cathelicidin antimicrobial peptide.
Spcs X-react.	Human, other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995). 2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995). 3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995). 4. Larrick J.W, et al. FEBS Lett. 398:74-80(1996). 5. Gudmundsson G.H, et al. Eur. J. Biochem. 238:325-332(1996). 6. Bals R, et al. Clin. Diagn. Lab. Immunol. 8:370-375(2001).
Limitation	For research use only

Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): whole serum

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: whole serum (OSC00037W), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.

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Associated Products

Code	Product Name
OSC00028G	Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG
OSC00038G	Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG
OSC00030P	Human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39)
GSC00002P	Antibacterial protein LL-37 control peptide
OSC00031P	Human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): FK peptide
OSC00032P	Human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): GK peptide
OSC00033P	Human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): KR peptide
OSC00034P	Human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): LL peptide