SEC22b antibody, SEC22L1 antibody

Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1): whole serum

$240USD

Code	OSS00040W
ID Tag	Rb326-200308-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from human Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24) conjugated to an immunogenic carrier protein was used as the antigen.
Conjugate	Unconjugated antibody
Accession	SEC22B_HUMAN SEC22B_RAT SEC22B_MOUSE
Also known as	SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, ERS24, ERS-24, Vesicle-trafficking protein SEC22b, SEC22L1, SEC22B
Background	The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript. FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex. SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Specific for SEC22B.
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Mao M, et al. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998). 2. Nakajima K, et al. EMBO J. 23:3216-3226(2004). 3. Chi A, et al. J. Proteome Res. 5:3135-3144(2006). 4. Matsuoka S, et al. Science 316:1160-1166(2007). 5. Xu D, et al. J. Biol. Chem. 275:39631-39639(2000). 6. Volchuk A, et al. Mol. Biol. Cell 15:1506-1518(2004). 7. Hay J.C, et al. Cell 89:149-158(1997). 8. Carninci P, et al. Science 309:1559-1563(2005). 9. Villen J, et al. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). 10. Gonzalez L.C. Jr, et al. J. Biol. Chem. 276:24203-24211(2001).
Limitation	For research use only

Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1): whole serum

IHC on rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat trigeminal using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

WB on rat brain using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1: 2000 dilution. DAPI counter stained.

IHC on rat spinal cord using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat brain using Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): whole serum (OSS00040W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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Associated Products

Code	Product Name
OSS00048G	Rabbit antibody to Vesicle-trafficking protein SEC22b (SEC22B, SEC22L1, ERS24): IgG