Rabbit antibody to Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1): whole serum

$240USD

Code	OSS00036W
ID Tag	Rb336-200308-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide as a part of human, rat, mouse, chimpanzee, xenopus, zebrafish (and other species) Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1) conjugated to an immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	VAMP7_HUMAN VAMP7_RAT VAMP7_MOUSE VAMP7_CHIMPANZEE VAMP7_FROG
Also known as	Vesicle-associated membrane protein 7, VAMP7, SYBL1, VAMP-7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP
Background	FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. SUBUNIT: Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type IV membrane protein. TISSUE SPECIFICITY: Detected in all tissues tested.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for VAMP7.
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. D'Esposito M, et al. Nat. Genet. 13:227-229(1996). 2. Ciccodicola A, et al. Hum. Mol. Genet. 9:395-401(2000). 3. Martinez-Arca S, et al. Proc. Natl. Acad. Sci. U.S.A. 100:9011-9016(2003). 4. Ward D.M, et al. Mol. Biol. Cell 11:2327-2333(2000). 5. Hibi T, et al. Biochem. Biophys. Res. Commun. 271:36-41(2000). 6. Siddiqi S.A, et al. J. Cell Sci. 119:943-950(2006). 7. Braun V, et al. EMBO J. 23:4166-4176(2004).
Limitation	For research use only

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1): whole serum (OSS00036W) at 1: 2000 dilution. DAPI counter stained.

IHC on rat spinal cord (ventral horn) using Rabbit antibody to Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1): whole serum (OSS00036W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat brain (hippocampus) using Rabbit antibody to Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1): whole serum (OSS00036W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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Associated Products

Code	Product Name
OSS00047G	Rabbit antibody to Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1): IgG