Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum

$240USD

Code	OSS00035W
ID Tag	Rb324-200308-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide as a part of human, rat, mouse, xenopus (and many other species) Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2) conjugated to an immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	VAMP2_HUMAN VAMP2_RAT VAMP2_MOUSE VAMP2_FROG
Also known as	VAMP-2, Synaptobrevin-2, VAMP2, SYB2
Background	The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for VAMP2.
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Archer B.T, et al. J. Biol. Chem. 265:17267-17273(1990). 2. Jagadish M.N, et al. Biochem. J. 317:945-954(1996). 3. Kutay U, et al. EMBO J. 14:217-223(1995). 4. Hanson M.A, et al. Nat. Struct. Biol. 7:687-692(2000). 5. Elferink L.A, et al. J. Biol. Chem. 264:11061-11064(1989). 6. Sutton R.B, et al. Nature 395:347-353(1998). 7. Martin L.B, et al. J. Biol. Chem. 273:1444-1452(1998).
Limitation	For research use only

Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (OSS00035W) at 1: 2000 dilution. DAPI counter stained.

WB on rat brain lysate using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (OSS00035W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide abolishes the detected band.

IHC on rat brain (cortex) using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (OSS00035W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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Associated Products

Code	Product Name
OSS00045G	Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): IgG