Rabbit antibody to Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1): whole serum

$240USD

Code	OSS00038W
ID Tag	Rb349-200308-WS
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Unit size	100 µl
Immunogen	A synthetic peptide as a part of rat, mouse Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1) conjugated to an immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	VAMP1_RAT VAMP1_MOUSE
Also known as	VAMP-1, Vamp1, Synaptobrevin-1, Syb1
Background	FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBUNIT: Interacts with VAPA and VAPB. SUBCELLULAR LOCATION: Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. SUBCELLULAR LOCATION: Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein. Note: Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. SUBCELLULAR LOCATION: Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. TISSUE SPECIFICITY: Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney, spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Specific for VAMP1.
Spcs X-react.	Rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Elferink L.A, et al. J. Biol. Chem. 264:11061-11064(1989). 2. Mandic R, et al. Gene 199:173-179(1997). 3. Prele C.M, et al. Exp. Cell Res. 282:24-34(2003). 4. Carninci P, et al. cience 309:1559-1563(2005). 5. Vosseller K, et al. Proteomics 5:388-398(2005).
Limitation	For research use only

WB on rat brain lysate using Rabbit antibody to Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1): whole serum (OSS00038W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected bands.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSS00044G	Rabbit antibody to Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1): IgG