OLIG2 antibody, Oligo2 antibody, RACK17 antibody

Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): whole serum

$240USD

Code	OSO00005W
ID Tag	Rb794-140209-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Conjugate	Unconjugated antibody
Accession	OLIG2_HUMAN OLIG2_RAT OLIG2_MOUSE
Also known as	Basic helix loop helix protein class B 1, BHLHB1, olig2, OLIGO2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C binding protein 2, Protein kinase C binding protein RACK17, RACK17
Background	Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. Subcellular location: Nucleus. Cytoplasm Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for OLIG2.
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Wang J, et al. Proc. Natl. Acad. Sci. U.S.A. 97:3497-3502(2000) 2. Ota T, et al. Nat. Genet. 36:40-45(2004) 3. Marie Y, et al. Lancet 358:298-300(2001) 4. Lu Q.R, et al. Proc. Natl. Acad. Sci. U.S.A. 98:10851-10856(2001)
Limitation	For research use only

WB on mouse brain lysate (denatured, reduced) using Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): whole serum (OSO00005W) at 1: 1000 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSO00008G	Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): IgG