A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Also known as
Basic helix loop helix protein class B 1, BHLHB1, olig2, OLIGO2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C binding protein 2, Protein kinase C binding protein RACK17, RACK17
Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Wang J, et al. Proc. Natl. Acad. Sci. U.S.A. 97:3497-3502(2000)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)
3. Marie Y, et al. Lancet 358:298-300(2001)
4. Lu Q.R, et al. Proc. Natl. Acad. Sci. U.S.A. 98:10851-10856(2001)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB, Flow Cyt. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for OLIG2.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.