OLIG2 antibody, Oligo2 antibody, RACK17 antibody

A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

OLIG2_HUMAN
OLIG2_RAT
OLIG2_MOUSE

Also known as

Basic helix loop helix protein class B 1, BHLHB1, olig2, OLIGO2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C binding protein 2, Protein kinase C binding protein RACK17, RACK17

Target

Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Transportation

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Wang J, et al. Proc. Natl. Acad. Sci. U.S.A. 97:3497-3502(2000)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)
3. Marie Y, et al. Lancet 358:298-300(2001)
4. Lu Q.R, et al. Proc. Natl. Acad. Sci. U.S.A. 98:10851-10856(2001)

Limitation

For research use only

Associated
products

Code	Product Name
OSO00008G	Rabbit antibody to OLIG2
OSO00032G	Sheep antibody to OLIG2
OSO00030W	Sheep antibody to OLIG2
OSO00054W	Rabbit antibody to OLIG2

Code

OSO00005W

$240USD

Unit size

100 µl

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB, Flow Cyt. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for OLIG2.

Spcs X-react.

Human, rat, mouse. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Code

OSO00005W

$240USD

PDF Data Sheet

- Click to download the Data Sheet

MSDS

Click to download the data sheet - Click to download the Material Safety Data Sheet

Code

OSO00005W

$240USD

WB on mouse brain lysate (denatured, reduced) using Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): whole serum (OSO00005W) at 1: 1000 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

IHC on rat spinal cord (paraffin section) using Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): whole serum (OSO00005W) at 1: 300 dilution incubated overnight at 4C.

Code

OSO00005W

$240USD

Ordering

Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors.
This item is in our stock. The expected delivery time is within 5 business days worldwide.

Products

Rabbit antibody to OLIG2

Checkout