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Code
OSN00001W
$240USD
ID Tag
Rb296-230208-WS
Immunogen
Native beta-nerve growth factor (Ngfb, beta-NGF) purified from mouse submandibular gland
Accession
Also known
Ngfb, beta-NGF
Target
NGF is a secreted molecule which plays an important roll in the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Manni L, et al. Neurosci. Lett. 426 (1), 39-44(2007).
Limitation
For research use only
Code
OSN00001W
$240USD
Unit size
150 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB, inhibition of biological activity. Tested in vitro, this antibody abolishes the neuronal survival and the outgrowth actions of murine NGF in chicken DRG. A dilution of 1 : 2000 is recommended for WB and IHC-P; for inhibition of biological activities a dilution of up to 1 : 100 is recommended and for in vivo 1-20 ul per gram body weight. The optimal dilution should be determined by the end user.
Specificity
Specific for NGF
Spcs X-react.
Mouse, rat and human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSN00001W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSN00001W
$240USD
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WB on rat salivary gland lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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Rabbit antibody to native NGF
