Synaptotagmin-1 antibody, Synaptotagmin1, SytI antibody,

Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum

$240USD

Code	OSS00022W
ID Tag	Rb315-200308-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from rat Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.
Conjugate	Unconjugated antibody
Accession	SYTI_RAT SYTI_MOUSE
Also known as	Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65
Background	FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for SYTI.
Spcs X-react.	Rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Schivell A.E, et al. J. Biol. Chem. 271:27770-27775(1996). 2. Coppola T, et al. J. Biol. Chem. 276:32756-32762(2001). 3. Heindel U, et al. FEBS Lett. 544:57-62(2003). 4. Schivell A.E, et al. Mol. Cell. Neurosci. 29:56-64(2005). 5. Sutton R.B, et al. Cell 80:929-938(1995). 6. Shao X, et al. Biochemistry 37:16106-16115(1998). 7. Fernandez I, et al. Neuron 32:1057-1069(2001).
Limitation	For research use only

WB on rat brain lysate using Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum (OSS00022W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum (OSS00022W) at 1: 2000 dilution. DAPI counter stained.

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Associated Products

Code	Product Name
OSS00141G	Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): IgG