Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum

$240USD

Code	OSC00024W
ID Tag	Rb317-200308-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide as a part of rat and mouse ChAT (choline O-acetyltransferase) conjugated to an immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	ChAT_RAT ChAT_MOUSE
Also known as	CHOACTase, choline acetylase, choline O-acetyltransferase, CMS1A, CMS1A2, B230380D24Rik
Background	Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for ChAT.
Spcs X-react.	Rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Ishii K, et al. Brain Res. Mol. Brain Res. 7:151-159(1990). 2. Kengaku M, et al. Brain Res. Mol. Brain Res. 18:71-76(1993). 3. Wu D, et al. J. Biol. Chem. 270:29111-29116(1995). 4. Govindasamy L, et al. J. Struct. Biol. 148:226-235(2004). 5. Cai Y, et al. EMBO J. 23:2047-2058(2004).
Limitation	For research use only

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum (OSC00024W) at 1: 2000 dilution. DAPI counter stained.

IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum (OSC00024W) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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OSC00008W	Rabbit antibody to human, rat, mouse ChAT (choline O-acetyltransferase): whole serum