| Code |
OSR00317W |
| ID Tag |
Rb791-140209-WS |
| Ordering info |
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This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
 |
| Unit size |
100 µl |
| Immunogen |
A synthetic peptide from the internal region of human Rho related GTP binding protein RhoU (CDC42 like GTPase 1, CDC42L1, Ryu GTPase) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 90% identity with rat and mouse sequence. |
| Conjugate |
Unconjugated antibody |
| Accession |
RHOU_HUMAN
RHOU_RAT
RHOU_MOUSE |
| Also known as |
Rho related GTP binding protein RhoU, RhoU, Ryu GTPase, CDC42-like GTPase 1, Rho GTPase-like protein ARHU, GTP-binding protein-like 1, ARHU, CDC42L1, G28K, WRCH1, Wnt-1 responsive Cdc42 homolog 1 |
| Background |
Function: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound.
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction › focal adhesion. Cell projection › podosome. Note: Localizes to podosomes in SRC-transformed cells.
Tissue specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta. |
| Host |
NZ white rabbit |
| Purity |
Whole serum |
| Clonality |
Polyclonal |
| Isotype |
Polyclonal, whole serum |
| Applications |
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Specificity |
Specific for RhoU. |
| Spcs X-react. |
Human, mouse, rat. Other species not yet tested. |
| Format |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Expiry Date |
12 months after reconstitution |
| Transportation |
This item will be shipped to you at ambient temperature in a lyophilised form. |
| References |
1. Tao W, et al. Genes Dev. 15:1796-1807(2001)
2. Bubb K.L, et al. Genetics 173:2165-2177(2006)
3. Ory S, et al. Biol. Cell 99:701-716(2007)
4. Ota T, et al. Nat. Genet. 36:40-45(2004)
5. Gregory S.G, et al. Nature 441:315-321(2006) |
| Limitation |
For research use only |
Primary Antibodies 
