A synthetic peptide from internal cytoplasmic region of human Zinc transporter 5 (SLC30A5, ZNT5) conjugated to an immunogenic carrier protein was used as the antigen.
Also known as
Solute carrier family 30 member 5, ZnT-like transporter 1, SLC30A5
FUNCTION: Functions as a zinc transporter. May be a transporter of zinc into beta cells in order to form insulin crystals. Partly regulates cellular zinc homeostasis. Required with ZNT7 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT6 and ZNT7 for the activation of TNAP.
SUBCELLULAR LOCATION: Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. INDUCTION: Increased intracellular zinc level, resulting from extracellular zinc supplementation, do not induce any up- or down-regulation of gene expression. Up-regulated by zinc depletion.
TISSUE SPECIFICITY: Ubiquitously expressed. Highly expressed in pancreas, liver and kidney. Expressed abundantly in insulin-containing beta cells, undetectable in other endocrine cell types including glucagon-secreting alpha cells and most acinar cells
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Kambe T, et al. J. Biol. Chem. 277:19049-19055(2002)
2. Cragg R.A, et al. J. Biol. Chem. 277:22789-22797(2002)
3. Devergnas S, et al. Biochem. Pharmacol. 68:699-709(2004)
4. Suzuki T, et al. J. Biol. Chem. 280:30956-30962(2005)
5. Suzuki T, et al. J. Biol. Chem. 280:637-643(2005)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for SLC30A5.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.