Rabbit antibody to human myeloperoxidase (MPO): IgG

$240USD

Code	DOM00001G
ID Tag	Rb431-200408-G
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	500 µg
Immunogen	Native myeloperoxidase isolated from human leucocytes
Conjugate	Unconjugated antibody
Accession	PERM_HUMAN
Also known as	MPO
Background	Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. SUBCELLULAR LOCATION: Lysosome
Host	NZ white rabbit
Purity	Protein G purified IgG
Clonality	Polyclonal
Isotype	Polyclonal, IgG
Applications	IHC, WB. A working concentration of 1-10 µg/ml is recommended. The optimal dilution should be determined by the end user. This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins.
Specificity	Appears to be specific for MPO.
Spcs X-react.	Human
Format	Lyophilised
Reconstitution	Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Morishita K, et al. J. Biol. Chem. 262:3844-3851(1987). 2. Johnson K.R, et al. Nucleic Acids Res. 15:2013-2028(1987). 3. Seto P, et al. J. Clin. Invest. 80:1205-1208(1987). 4. Hashinaka K, et al. Biochemistry 27:5906-5914(1988). 5. Hosokawa Y, et al. Leukemia 7:441-445(1993). 6. Yamada M, et al. J. Biol. Chem. 268:13479-13485(1993). 7. Liu T, et al. J. Proteome Res. 4:2070-2080(2005). 8. Ramachandran P, et al. J. Proteome Res. 5:1493-1503(2006). 9. Fiedler T.J, et al. J. Biol. Chem. 275:11964-11971(2000). 10. Sjoeblom T, et al. Science 314:268-274(2006).
Limitation	For research use only

Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to human myeloperoxidase (MPO): IgG (3µg/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to human myeloperoxidase (MPO): IgG (3µg/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

10 μg of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to human myeloperoxidase (MPO): IgG (3 µg/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to human myeloperoxidase (MPO): red; Hoechst: blue; MHC class II: green. Magnification: 200 X

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