ATG4B antibody, APG4B antibody

Rabbit antibody to human, mouse cysteine protease ATG4B (autophagin-1, APG4B, AUTL1): whole serum

$240USD

Code	OSA00033W
ID Tag	Rb270-270108-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	150 µl
Immunogen	A synthetic peptide from human cysteine protease ATG4B (autophagin-1, APG4B, AUTL1) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse.
Conjugate	Unconjugated antibody
Accession	ATG4B_HUMAN ATG4B_MOUSE
Also known as	APG4B, AUTL1, hAPG4B, Autophagin-1, AUTL1, MGC1353, KIAA0943, cysteine protease ATG4B, hAPG4B, AUT-like 1 cysteine endopeptidase, Autophagy-related protein 4 homolog B
Background	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide SUBCELLULAR LOCATION: Cytoplasm
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for ATG4B.
Spcs X-react.	Human, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Marino G, et al. J. Biol. Chem. 278:3671-3678(2003). 2. Olsen J.V, et al. Cell 127 (3), 635-648(2006). 3. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006). 4. Kumanomidou T, et al. J. Mol. Biol. 355 (4), 612-618(2006). 5. Tanida I, et al. J. Biol. Chem. 279 (46), 47704-47710(2004). 6. Kabeya Y, et al. J. Cell Sci. 117:2805-2812(2004).
Limitation	For research use only

WB on human neutrophil lysate using Rabbit antibody to human, mouse cysteine protease ATG4B (autophagin-1, APG4B, AUTL1): whole serum (OSA00033W) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

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MSDS	- Click to download the Material Safety Data Sheet

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Code	Product Name
OSA00038W	Sheep antibody to human, mouse cysteine protease ATG4B (autophagin-1, APG4B, AUTL1): whole serum