contactin-1 antibody, contactin 1 antibody

Rabbit antibody to contactin-1 (contactin 1, CNTN1): whole serum

$240USD

Code	OSC00027W
ID Tag	Rb430-200408-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from human contactin-1 (F3cam, contactin 1, CNTN1) conjugated to an immunogenic carrier protein was used as the antigen.
Conjugate	Unconjugated antibody
Accession	CNTN1_HUMAN CNTN1_RAT
Also known as	Neural cell surface protein F3, F3cam, contactin 1, CNTN1, Glycoprotein gp135
Background	FUNCTION: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. MISCELLANEOUS: F3 shares with L1, N-CAM, MAG, and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. TISSUE SPECIFICITY: Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for contactin-1.
Spcs X-react.	Human, rat. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Reid R.A, et al. Brain Res. Mol. Brain Res. 21:1-8(1994). 2. Berglund E.O, et al. Genomics 21:571-582(1994). 3. Ota T, et al. Nat. Genet. 36:40-45(2004). 4. Berglund E, et al. Eur. J. Biochem. 197:549-554(1991). 5. Liu T, et al. J. Proteome Res. 4:2070-2080(2005). 6. Sjoeblom T, et al. Science 314:268-274(2006).
Limitation	For research use only

IHC on rat spinal cord (free floating section) using Rabbit antibody to contactin-1 (contactin 1, CNTN1): whole serum (OSC00027W) at 1 : 300 dilution incubated overnight at room temperature (21C) developed with DAB/Ni.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSC00195G	Rabbit antibody to contactin-1 (contactin 1, CNTN1): IgG
OSC00026W	Rabbit antibody to mouse contactin-1 (Contactin 1, CNTN1): whole serum