Code
OSC00028G
$240USD    Buy Now 

ID Tag
Rb259-270108-G

Immunogen
A synthetic peptide from human Cathelicidin antimicrobial peptide conjugated to an immunogenic carrier protein has been used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

Target
Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995).
2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995).
3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995).

Limitation
For research use only

Related
products

Code
OSC00028G
$240USD    Buy Now 

Unit size
500 g

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. Use at a concentration of 10-50 g/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Cathelicidin antimicrobial peptide.

Spcs X-react.
Human, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00028G
$240USD    Buy Now 


Rabbit antibody to LL-37 Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to LL-37 (OSC00028G), diluted 50 µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI.  The antibody selectively recognizes polymorphonuclar cells.
Rabbit antibody to LL-37 WB on human neutrophil lysate using Rabbit antibody to LL-37 (OSC00028G) at 50 g/ml concentration. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
Products
Rabbit antibody to LL-37
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