CAP-18 antibody, LL37 antibody, CAMP antibody, CRAMP antibody

Rabbit antibody to LL-37

$240USD

Code	OSC00028G
ID Tag	Rb259-270108-G
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The expected delivery time is within 5 business days worldwide.
Unit size	500 µg
Immunogen	A synthetic peptide from human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39) conjugated to an immunogenic carrier protein has been used as the antigen. The location of the epitope is highlighted in green.
Conjugate	Unconjugated antibody
Accession	CAMP_HUMAN
Also known as	CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26
Target	Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.
Host	NZ white rabbit
Purity	IgG
Clonality	Polyclonal
Isotype	Polyclonal, IgG
Applications	IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for Cathelicidin antimicrobial peptide.
Spcs X-react.	Human, other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995). 2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995). 3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995). 4. Larrick J.W, et al. FEBS Lett. 398:74-80(1996). 5. Gudmundsson G.H, et al. Eur. J. Biochem. 238:325-332(1996). 6. Bals R, et al. Clin. Diagn. Lab. Immunol. 8:370-375(2001).
Limitation	For research use only

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18): IgG (OSC00028G), diluted 50 µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.

WB on human neutrophil lysate using Rabbit antibody to human Cathelicidin antimicrobial peptide (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG (OSC00028G) at 50 µg/ml concentration. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSC00155G	Mouse monoclonal to Cathelicidin: OSX12 clone
OSC00067W	Rabbit antibody to CAP-18
OSC00009W	Rabbit antibody to LL-37
OSC00066W	Rabbit antibody to CAP-18
OSC00039W	Rabbit antibody to scrambled Cathelicidin
OSC00038G	Sheep antibody to LL-37
OSC00037W	Sheep antibody to LL-37
OSC00071G	Rabbit antibody to CAP-18