Code
OSC00028G
$240USD    Buy Now 

ID Tag
Rb259-270108-G

Immunogen
A synthetic peptide from human Cathelicidin antimicrobial peptide conjugated to an immunogenic carrier protein has been used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

Target
Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995).
2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995).
3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995).

Limitation
For research use only

Related
products

Code
OSC00028G
$240USD    Buy Now 

Unit size
500 g

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. Use at a concentration of 10-50 g/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Cathelicidin antimicrobial peptide.

Spcs X-react.
Human, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Note
Control column is available at $80 per kit. Please enquire sales@osenses.com

Description
Osenses Control column kit offers a convenient way to perform an On-Column-Absorption (OCA). In OCA both the bound antibody and the flow-through is utilized. The bound immunoglobulin is the antigen specific antibody and the flow-through is the negative control. OCA is an effective alternative to affinity purification and absorption at the same time.

Items Provided
- 1x OS-50 mini spin column with Luer cap and a lid
- 20 μg control peptide
- 50 μl activated beads

Items Required
- Microtubes
- Syringe or microcentrifuge
- Sodium bicarbonate buffer (100 mM, pH: 10)
- Sodium borohydride
- PBS
- Sodium azide
Protocol

Coupling buffer: 100 mM sodium bicarbonate

Component

FW

Amount

Final Concentration

pH

MQ H2O

20 ml

10

NaHCO3

84.0

0.42 g

100 mM

Adjust the pH to 10 with NaOH.

MQ H2O

Up to 50 ml

Protocol for coupling of the control peptide to the beads

Wash>Conjugate>Use immediately or Store

Hands-on work: 15 minutes

1. Dissolve completely the peptide in 50 µl MQ water and put aside.

2. Wash the activated beads in the OS-50 spin column with MQ water. First, remove the outlet cap from the column and then place the column in a microtube.

3. Add 450 µl MQ water to the column making sure that all the beads are collected to the bottom of the tube. Discard the flow through. Repeat the washing step two more times with water. Note that the washing can also be done using the Luer cap provided and a syringe or a centrifuge (just pulse spin).

4. Cap the outlet of the spin column and add 450 µl of coupling buffer.

5. Add the peptide solution to the column and incubate for 4 h at RT (23C) or overnight at 4C while gently mixing.

6. Add 0.5 mg sodium borohydride and incubate for 30 min at RT.

7. Open the outlet cap. Wash with 450 µl of PBS pH 7. Wash two more times with 450 µl PBS.

8. The column is ready. Use immediately or add 200 µl of PBS containing 0.1% NaN3 and store at 4C.


TBS buffer

Component

FW

Amount

Final Concentration

pH

MQ H2O

50 ml

7.4

Sigma 7-9 (equivalent to Tris base, T1378-1Kg)

121.14

0.6 g

50 mM

NaCl

58.44

0.9

154 mM

Adjust the pH to 7.4 with HCl.

MQ H2O

Up to 100 ml

Glycine solution

Component

FW

Amount

Final Concentration

pH

MQ H2O

20 ml

2.5

Glycine

75.07

0.375 g

0.1 M

Adjust the pH to 2.5 with HCl.

MQ H2O

Up to 50 ml

Protocol for On-Column-Absorption (OCA)

Wash the column> Equilibrate the column>Dilute the sample in TBS>Apply to the column>Incubate>Wash>Keep the flow thru>Elute and keep the Eluate “Antigen specific antibody”

Hands-on work: 30 minutes



Code
OSC00028G
$240USD    Buy Now 


Rabbit antibody to LL-37 Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to LL-37 (OSC00028G), diluted 50 µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI.  The antibody selectively recognizes polymorphonuclar cells.
Rabbit antibody to LL-37 WB on human neutrophil lysate using Rabbit antibody to LL-37 (OSC00028G) at 50 g/ml concentration. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
Products
Rabbit antibody to LL-37
Content

Download Protocols

IHC, Paraffin section
APES coating of slides