Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum

$240USD

Code	OSS00010W
ID Tag	Rb305-200308-WS
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Unit size	100 µl
Immunogen	A synthetic peptide as a part of extracellular, N-terminal part of rat Sortilin (Neurotensin receptor 3, NTR3, Sort1) conjugated to immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	SORT_RAT
Also known as	Neurotensin receptor 3, NTR3, Glycoprotein 110, Gp110, Nt3, Nts3
Background	FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP and GM2A, LPL, PSAP and SLC2A4. Forms a complex with NGFR which binds specifically to the precursor form of NGFB (proNGFB). Interacts with the precursor form of BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note: Localized to membranes of the endosomes, Golgi stack, lysosomes and nucleus. Localization to the plasma membrane in adipocytes is enhanced by insulin. Localized to the endoplasmic reticulum and the plasma membrane. Also found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. TISSUE SPECIFICITY: Highly expressed in fat, brain, and lung. Expressed in neuronal bodies and dendrites of the piriform cortex and hippocampus. Also expressed in the islands of Calleja, medial and lateral septal nuclei, amygdaloid nuclei, thalamic nuclei, the supraoptic nucleus, the substantia nigra, the Purkinje layer of the cerebellar cortex and the cranial motor nerve nuclei of the brainstem.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for Sortilin.
Spcs X-react.	Rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Gibbs R.A, et al. Nature 428:493-521(2004). 2. Morris N.J, et al. J. Biol. Chem. 273:3582-3587(1998). 3. Lin B.-Z, et al. J. Biol. Chem. 272:24145-24147(1997). 4. Conticello S.G, et al. J. Biol. Chem. 278:26311-26314(2003). 5. Sarret P, et al. J. Comp. Neurol. 461:483-505(2003). 6. Chen Z.-Y, et al. J. Neurosci. 25:6156-6166(2005).
Limitation	For research use only

Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (OSS00010W) at 1: 2000 dilution. DAPI counter stained.

WB on rat brain lysate using Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (OSS00010W) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

IHC on rat brain using Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (OSS00010W) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

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Code	Product Name
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