STIM1 antibody, Stromal interaction molecule 1 antibody, GOK antibody

Rabbit antibody to extracellular domain of Stromal interaction molecule 1 (STIM1, GOK): whole serum

$240USD

Code	OSS00139W
ID Tag	Rb740-061208-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from the extracellular domain of human Stromal interaction molecule 1 (STIM1) conjugated to an immunogenic carrier protein was used as the immunogen.
Conjugate	Unconjugated antibody
Accession	STIM1_HUMAN STIM1_RAT STIM1_MOUSE
Also known as	STIM1, GOK
Background	FUNCTION: Plays a role in mediating Ca²⁺ influx following depletion of intracellular Ca²⁺ stores. Acts as Ca²⁺ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca²⁺ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca²⁺ release-activated Ca²⁺ (CRAC) channel subunit, TMEM142A/ORAI1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Tissue specificity: Ubiquitously expressed in various human primary cells and tumor cell lines.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.
Specificity	Specific for STIM1.
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Parker N.J, et al. Genomics 37:253-256(1996) 2. Sabbioni S, et al. Cancer Res. 57:4493-4497(1997) 3. Manji S.S, et al. Biochim. Biophys. Acta 1481:147-155(2000) 4. Williams R.T, et al. Biochem. J. 357:673-685(2001) 5. Chen R, et al. J. Proteome Res. 8:651-661(2009)
Limitation	For research use only

Rabbit antibody to extracellular domain of Stromal interaction molecule 1 (STIM1, GOK): whole serum

WB on mouse brain lysate (denatured, reduced) using Rabbit antibody to extracellular domain of Stromal interaction molecule 1 (STIM1, GOK): whole serum (OSS00139W) at 1: 1000 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSS00140G	Rabbit antibody to extracellular domain of Stromal interaction molecule 1 (STIM1, GOK): IgG