VGLUT1 antibody, Vesicular glutamate transporter 1 antibody, Slc17a7 antibody

Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Vesicular glutamate transporter 1): whole serum

$240USD

Code	OSV00006W
ID Tag	Rb428-060408-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from a cytoplasmic loop of rat VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1) conjugated to an immunogenic carrier protein was used as the antigen.
Conjugate	Unconjugated antibody
Accession	VGLUT1_RAT VGLUT1_MOUSE VGLUT1_HUMAN
Also known as	Vesicular glutamate transporter 1, VGlU1, Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Bnpi
Background	FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB, Flow Cyt. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for VGLUT1 (both isoforms 1 and 2).
Spcs X-react.	Human, rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Ni B, et al. Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994). 2. Nogami H, et al. J. Neurochem. 99:142-153(2006). 3. Takamori S, et al. Nature 407:189-194(2000). 4. Bellocchio E.E, et al. Science 289:957-960(2000). 5. Herzog E, et al. J. Neurosci. 21:RC181-RC181(2001). 6. Takamori S, et al. J. Neurosci. 21:RC182-RC182(2001). 7. Fremeau R.T, et al. Neuron 31:247-260(2001). 8. Gras C, et al. Nat. Neurosci. 11:292-300(2008). 9. Takamori S, et al. Cell 127:831-846(2006). 10. Mackenzie B, et al. Neurochem. Res. 33:238-247(2008).
Limitation	For research use only

Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Vesicular glutamate transporter 1): whole serum

WB on rat brain lysate using Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1): whole serum (OSV00006W) at 1 : 2000 dilution.

IHC on rat brain (free floating sections) using Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1): whole serum (OSV00006W) at 1 : 1000 dilution.

IHC on rat spinal cord (paraffin section) using Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1): whole serum (OSV00006W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.

IHC on rat spinal cord (free floating sections) using Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Vesicular glutamate transporter 1): whole serum (OSV00006W) at 1 : 300 dilution incubated overnight at room termperature and developed with DAB/Ni.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

Associated Products

Code	Product Name
OSV00007G	Rabbit antibody to a cytoplasmic loop of VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1): IgG