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Code
OSR00136W
$240USD    Buy Now 

ID Tag
Rb131-281007-WS

Immunogen
A synthetic peptide from aa region 300-400 of mouse TRPV4 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and human.
The location of the epitope is highlighted in green.

Accession

Also known
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, Trp12, Vrl2

Target
TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.
FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Liedtke W.B, et al. Cell 103:525-535(2000).
2. Wissenbach U, et al. FEBS Lett. 485:127-134(2000).
3. Strotmann R, et al. Nat. Cell Biol. 2:695-702(2000).

Limitation
For research use only

Related
products

Code
OSR00136W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other application.

Specificity
Specific for TRPV4.

Spcs X-react.
Mouse, human and rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Code
GST00018P
$120USD   

ID Tag
40564-27

Unit size
50 g

Accession

Also known
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2

Target
This is the immunising peptide for the products Rabbit antibody to TRPV4 (OSR00136W), Sheep antibody to TRPV4 (OST00035W), Rabbit antibody to TRPV4 (OST00265G)

Purity
>84%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSR00136W,OST00035W,OST00265G

Note
Control column is available at $80 per kit. Please enquire sales@osenses.com

Description
Osenses Control column kit offers a convenient way to perform an On-Column-Absorption (OCA). In OCA both the bound antibody and the flow-through is utilized. The bound immunoglobulin is the antigen specific antibody and the flow-through is the negative control. OCA is an effective alternative to affinity purification and absorption at the same time.

Items Provided
- 1x OS-50 mini spin column with Luer cap and a lid
- 20 μg control peptide
- 50 μl activated beads

Items Required
- Microtubes
- Syringe or microcentrifuge
- Sodium bicarbonate buffer (100 mM, pH: 10)
- Sodium borohydride
- PBS
- Sodium azide
Protocol

Coupling buffer: 100 mM sodium bicarbonate

Component

FW

Amount

Final Concentration

pH

MQ H2O

20 ml

10

NaHCO3

84.0

0.42 g

100 mM

Adjust the pH to 10 with NaOH.

MQ H2O

Up to 50 ml

Protocol for coupling of the control peptide to the beads

Wash>Conjugate>Use immediately or Store

Hands-on work: 15 minutes

1. Dissolve completely the peptide in 50 µl MQ water and put aside.

2. Wash the activated beads in the OS-50 spin column with MQ water. First, remove the outlet cap from the column and then place the column in a microtube.

3. Add 450 µl MQ water to the column making sure that all the beads are collected to the bottom of the tube. Discard the flow through. Repeat the washing step two more times with water. Note that the washing can also be done using the Luer cap provided and a syringe or a centrifuge (just pulse spin).

4. Cap the outlet of the spin column and add 450 µl of coupling buffer.

5. Add the peptide solution to the column and incubate for 4 h at RT (23C) or overnight at 4C while gently mixing.

6. Add 0.5 mg sodium borohydride and incubate for 30 min at RT.

7. Open the outlet cap. Wash with 450 µl of PBS pH 7. Wash two more times with 450 µl PBS.

8. The column is ready. Use immediately or add 200 µl of PBS containing 0.1% NaN3 and store at 4C.


TBS buffer

Component

FW

Amount

Final Concentration

pH

MQ H2O

50 ml

7.4

Sigma 7-9 (equivalent to Tris base, T1378-1Kg)

121.14

0.6 g

50 mM

NaCl

58.44

0.9

154 mM

Adjust the pH to 7.4 with HCl.

MQ H2O

Up to 100 ml

Glycine solution

Component

FW

Amount

Final Concentration

pH

MQ H2O

20 ml

2.5

Glycine

75.07

0.375 g

0.1 M

Adjust the pH to 2.5 with HCl.

MQ H2O

Up to 50 ml

Protocol for On-Column-Absorption (OCA)

Wash the column> Equilibrate the column>Dilute the sample in TBS>Apply to the column>Incubate>Wash>Keep the flow thru>Elute and keep the Eluate “Antigen specific antibody”

Hands-on work: 30 minutes



Code
OSR00136W
$240USD    Buy Now 


Rabbit antibody to TRPV4 (300-400) WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 (OSR00136W) at 1 : 1000 dilution.

Products
Rabbit antibody to TRPV4 (300-400)