A synthetic peptide from aa region 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
The location of the epitope is highlighted in
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, Trp12, Vrl2
TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.
FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Liedtke W.B, et al. Cell 103:525-535(2000).
2. Wissenbach U, et al. FEBS Lett. 485:127-134(2000).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other application.
Specific for TRPV4.
Mouse, human, rat, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.