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Code
OSR00136W
$240USD    Buy Now 

ID Tag
Rb131-281007-WS

Immunogen
A synthetic peptide from aa region 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
The location of the epitope is highlighted in green.

Accession

Also known
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, Trp12, Vrl2

Target
TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.
FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Liedtke W.B, et al. Cell 103:525-535(2000).
2. Wissenbach U, et al. FEBS Lett. 485:127-134(2000).

Limitation
For research use only

Related
products

Code
OSR00136W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other application.

Specificity
Specific for TRPV4.

Spcs X-react.
Mouse, human and rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Code
GST00018P
$120USD   

ID Tag
40564-27

Unit size
50 g

Accession

Also known
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2

Target
This is the immunising peptide for the products Rabbit antibody to TRPV4 (OSR00136W), Sheep antibody to TRPV4 (OST00035W), Rabbit antibody to TRPV4 (OST00265G)

Purity
>84%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSR00136W,OST00035W,OST00265G

Code
OSR00136W
$240USD    Buy Now 


Rabbit antibody to TRPV4 (300-400) WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 (OSR00136W) at 1 : 1000 dilution.
Rabbit antibody to TRPV4 (300-400) IHC on paraffin sections of rat kidney using Rabbit antibody to TRPV4 (300-400): OSR00136W.
Animal was perfused with Autoperfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module
Detection was done using Novolink HRP polymer from Leica following manufactures instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Rabbit antibody to TRPV4 (300-400) IHC on paraffin sections of rat spleen using Rabbit antibody to TRPV4 (300-400): OSR00136W.
Animal was perfused with Autoperfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module
Detection was done using Novolink HRP polymer from Leica following manufactures instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Rabbit antibody to TRPV4 (300-400) IHC on paraffin sections of rat spleen using Rabbit antibody to TRPV4 (300-400): OSR00136W.
Animal was perfused with Autoperfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module
Detection was done using Novolink HRP polymer from Leica following manufactures instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Rabbit antibody to TRPV4 (300-400) IHC on paraffin sections of rat kidney using Rabbit antibody to TRPV4 (300-400): OSR00136W.
Animal was perfused with Autoperfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module
Detection was done using Novolink HRP polymer from Leica following manufactures instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Products
Rabbit antibody to TRPV4 (300-400)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer (Reducing)
IHC-P on Autostainer
RIPA Buffer
WB Protocol online
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