Rabbit antibody to 1t cytoplasmic loop of mouse, human, rat TRPV4 (VRL-2): whole serum

$240USD

Code	OST00199W
ID Tag	Rb620-170808-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from the 1t cytoplasmic loop of mouse, human, rat TRPV4 (VRL-2) conjugated to an immunogenic carrier protein was used as the immunogen. The location of the epitope is highlighted in green.
Conjugate	Unconjugated antibody
Accession	TRPV4_MOUSE TRPV4_HUMAN TRPV4_RAT
Also known as	Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, VR-OAC, MGC150050, 0610033B08Rik, Trp12, Vrl2, Vroac
Background	TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity	Appears to be specific for TRPV4.
Spcs X-react.	Mouse, human and rat. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Liedtke W.B, et al. Cell 103:525-535(2000). 2. Wissenbach U, et al. FEBS Lett. 485:127-134(2000). 3. Strotmann R, et al. Nat. Cell Biol. 2:695-702(2000). 4. Earley,S, et al. Circ. Res. 97 (12), 1270-1279 (2005). 5. Arniges M, et al. J. Biol. Chem. 281:1580-1586(2006). 6. Strotmann R, et al. J. Biol. Chem. 278:26541-26549(2003).
Limitation	For research use only

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