KCNJ12 antibody, KCNJN1 antibody, Kir2.2 antibody, IRK2 antibody, ATP-sensitive inward rectifier potassium channel 12 antibody

Rabbit antibody to cytoplasmic domain of KCNJ12 (KCNJN1, Kir2.2, IRK2): whole serum

$240USD

Code	OSK00021W
ID Tag	Rb859-290309-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from the cytoplasmic domain of rat KCNJ12 (KCNJN1, Kir2.2, IRK2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.
Conjugate	Unconjugated antibody
Accession	KCNJ12_RAT KCNJ12_MOUSE
Also known as	ATP-sensitive inward rectifier potassium channel 12, Potassium channel, inwardly rectifying subfamily J member 12, Inward rectifier K(+) channel Kir2.2, Kir2.2v, IRK2, KCNJN1
Background	FUNCTION: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas. Subcellular location: Membrane, Multi-pass membrane protein.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for KCNJ12.
Spcs X-react.	Rat, mouse. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Koyama H, et al. FEBS Lett. 341:303-307(1994) 2. Leonoudakis D, et al. J. Biol. Chem. 279:19051-19063(2004)
Limitation	For research use only

Rabbit antibody to cytoplasmic domain of KCNJ12 (KCNJN1, Kir2.2, IRK2): whole serum (OSK00021W) at 1: 1000 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

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Associated Products

Code	Product Name
OSK00028G	Rabbit antibody to cytoplasmic domain of KCNJ12 (KCNJN1, Kir2.2, IRK2): IgG