A synthetic peptide from aa region 640-680 of human Tau (Microtubule associated protein tau) conjugated to an immunogenic carrier protein was used as the antigen.
Also known as
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MAPTL, MTBT1
Function: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Subcellular location: Cytoplasm › cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Cell projection › axon. Note: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Tissue specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Goedert M, et al. Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055(1988)
2. Andreadis A, et al. Biochemistry 31:10626-10633(1992)
3. Cripps D, et al. J. Biol. Chem. 281:10825-10838(2006)
4. Mori H, et al. Biochem. Biophys. Res. Commun. 159:1221-1226(1989)
5. Zarranz J.J, et al. Neurology 64:1578-1585(2005)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Tau.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.