VGLUT3 antibody

Rabbit antibody to c-terminal region of VGluT3 (Vesicular glutamate transporter 3): whole serum

$240USD

Code	OSV00047W
ID Tag	Rb848-290309-WS
Ordering info	Order online using the shopping cart ("Add to Cooler"), by email to sales@osenses.com or fax to 61-8-827 0 8586 and pay via PayPal by MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors. This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
Unit size	100 µl
Immunogen	A synthetic peptide from the c-terminal region of mouse VGLUT3 conjugated to an immunogenic carrier protein was used as the antigen.
Conjugate	Unconjugated antibody
Accession	VGLUT3_MOUSE VGLUT3_RAT
Also known as	Vesicular glutamate transporter 3, Solute carrier family 17 member 8
Background	FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Membrane; Multi-pass membrane proteinPotential. Cell junction › synapse › synaptosome. TISSUE SPECIFICITY: Expressed in brain, kidney and liver. Expressed within the amygdala, brainstem, cerberal cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, hypothalamus, retina, striatum and ventral spinal cord. Expressed within neurons of the caudate-putamen, olfactory tubercle, nucleus accumbens, hippocampus, interpeduncular nucleus and dorsal and medial raphe nuclei. Expressed in inner hair cells of the ear. Expressed at synaptic terminals within the lateral superior olive (LSO), a nucleus of the mammalian sound localization system, and in the medial nucleus of the trapezoid body (MNTB), which provides inhibitory input to the LSO.
Host	NZ white rabbit
Purity	Whole serum
Clonality	Polyclonal
Isotype	Polyclonal, whole serum
Applications	IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity	Specific for VGLUT3.
Spcs X-react.	Mouse, rat. Other species not yet tested.
Format	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date	12 months after reconstitution
Transportation	This item will be shipped to you at ambient temperature in a lyophilised form.
References	1. Schaefer M.K.-H, et al. J. Biol. Chem. 277:50734-50748(2002) 2. Ruel J, et al. Am. J. Hum. Genet. 83:278-292(2008) 3. Gras C, et al. Nat. Neurosci. 11:292-300(2008) 4. Mackenzie B, et al. Neurochem. Res. 33:238-247(2008) 5. Seal R.P, et al. Neuron 57:263-275(2008)
Limitation	For research use only

Rabbit antibody to c-terminal region of VGluT3 (Vesicular glutamate transporter 3): whole serum

WB on mouse brain lysate (10% gel, 1:29; reduced, denatured) using Rabbit antibody to c-terminal region of mouse VGluT3 (Vesicular glutamate transporter 3): whole serum (OSV00047W) at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (0.1 x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

IHC on rat spinal cord (free floating sections) using Rabbit antibody to c-terminal region of VGluT3 (Vesicular glutamate transporter 3): whole serum (OSV00047W) at 1 : 300 dilution incubated overnight at room temperature and developed with DAB/Ni.

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Associated Products

Code	Product Name
OSV00048G	Rabbit antibody to c-terminal region of mouse VGluT3 (Vesicular glutamate transporter 3): IgG