| Code |
OSP00056W |
| ID Tag |
Rb897-260409-WS |
| Ordering info |
Order online using the shopping cart ("Add to Cooler"), by email to
sales@osenses.com
or fax to 61-8-827 0 8586 and pay via PayPal by
MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors.
This item is in our stock. The handling & shipping cost is $48 USD and the expected delivery time is within 5 business days worldwide.
 |
| Unit size |
100 µl |
| Immunogen |
A synthetic peptide from c-terminal region (~aa 100-170) of human p27 Kip1 (KIP1, p27Kip1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Conjugate |
Unconjugated antibody |
| Accession |
p27Kip1_HUMAN
p27Kip1_RAT
p27Kip1_MOUSE |
| Also known as |
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B, KIP1, p27Kip1, p27 Kip 1 |
| Background |
FUNCTION: Important regulator of cell cycle progrssion. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originating from lung, breast, colon, ovary, esophagus, thyroid and prostate.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Nuclear and cytoplasmic in quiescent cells.
Tissue specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. |
| Host |
NZ white rabbit |
| Purity |
Whole serum |
| Clonality |
Polyclonal |
| Isotype |
Polyclonal, whole serum |
| Applications |
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Specificity |
Specific for p27Kip1. |
| Spcs X-react. |
Human, rat, mouse. Other species not yet tested. |
| Format |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Expiry Date |
12 months after reconstitution |
| Transportation |
This item will be shipped to you at ambient temperature in a lyophilised form. |
| References |
1. Polyak K, et al. Cell 78:59-66(1994)
2. Pietenpol J.A, et al. Cancer Res. 55:1206-1210(1995)
3. Ishida N, et al. J. Biol. Chem. 275:25146-25154(2000)
4. Viglietto G, et al. Nat. Med. 8:1136-1144(2002)
5. Grimmler M, et al. Cell 128:269-280(2007) |
| Limitation |
For research use only |
Primary Antibodies 
