A synthetic peptide from the c-terminal region of mouse HGS (Hepatocyte growth factor-regulated tyrosine kinase substrate) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in human and rat.
Hepatocyte growth factor-regulated tyrosine kinase substrate, HRS
FUNCTION: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Subcellular location: Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome › multivesicular body membrane; Peripheral membrane protein
Tissue specificity: Ubiquitous expression in adult and fetal tissues with higher expression in testis.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Asao H, et al. J. Biol. Chem. 272:32785-32791(1997)
2. Rikova K, et al. Cell 131:1190-1203(2007)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for HGS.
Human, rat mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.