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Code
OSA00019W
$240USD
ID Tag
Rb286-270108-WS
Immunogen
A synthetic peptide from c-terminal region of human autophagy-related protein 7 (ATG7) conjugated to an immunogenic carrier protein has been used as the antigen.
Accession
Also known as
ATG7, APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein
Target
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
Isotype
Polyclonal, whole serum
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Transportation
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).
2. Nemoto T, et al. J. Biol. Chem. 278 (41), 39517-39526(2003).
3. Mizushima N, et al. FEBS Lett. 532 (3), 450-454(2002).
4. Tanida I, et al. J. Biol. Chem. 277 (16), 13739-13744(2002).
2. Nemoto T, et al. J. Biol. Chem. 278 (41), 39517-39526(2003).
3. Mizushima N, et al. FEBS Lett. 532 (3), 450-454(2002).
4. Tanida I, et al. J. Biol. Chem. 277 (16), 13739-13744(2002).
Limitation
For research use only
Associated
products
products
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Code
OSA00019W
$240USD
Unit size
150 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC. Use at a dilution of 1 : 100 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ATG7.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Transportation
This item will be shipped to you at ambient temperature in a lyophilised form.
Code
OSA00019W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00019W
$240USD
Ordering
Order online using the shopping cart ("Add to Cooler"), by email to
sales@osenses.com
or fax to 61-8-827 0 8586 and pay via PayPal by
MasterCard or Visa. You can also provide us with your Purchase Order Number (PON) and pay by check or wire transfer. Alternatively contact our distributors.
This item is in our stock. The expected delivery time is within 5 business days worldwide.
This item is in our stock. The expected delivery time is within 5 business days worldwide.
Products
| Rabbit antibody to ATG7 |
