A synthetic peptide from human VACHT conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Also known as
Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3, MGC12716
FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.
VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle.
SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Ichikawa T, et al. J Chem Neuroanat. 13(1):23-39(1997).
2. Roghani A, et al. Proc. Natl. Acad. Sci. U.S.A. 91:10620-10624(1994).
3. Erickson J.D, et al. J. Biol. Chem. 269:21929-21932(1994).
4. Bejanin S, et al. J. Biol. Chem. 269:21944-21947(1994).
For research use only
Polyclonal, whole serum
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for VACHT.
Human, mouse, rat. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.