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Code
OST00061W
$240USD    Buy Now 

ID Tag
Rb432-150608-WS

Immunogen
A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
The location of the epitope is highlighted in green.

Accession

Also known
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1

Target
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
TISSUE SPECIFICITY: Expressed in inner ear. In inner ear, it is found in the distal half of stereocilia, along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.
DEVELOPMENTAL STAGE: During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.
Inhibited by ruthenium red, a potent blocker of TRPV channels.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Jordt S.-E, et al. Nature 427:260-265(2004).

Limitation
For research use only

Related
products
 
Code Product Name
OST00062G Rabbit antibody to TRPA1
 

Code
OST00061W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 6000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, tissue lysates should be prepared in G3 lysing buffer (G3 lysing buffer contains only 1% Triton X-100 and no other detergents).

Specificity
Specific for TRPA1.

Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Code
GST00025P
$120USD   

ID Tag
50278-50

Unit size
50 g

Accession

Also known
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1

Target
This is the immunising peptide for the products Rabbit antibody to TRPA (OST00061W), Rabbit antibody to TRPA1 (OST00062G), Sheep antibody to TRPA1 (OST00289W), Sheep antibody to TRPA1 (OST00290G)

Purity
>87%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in water.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OST00061W,OST00062G,OST00289W,OST00290G

Code
OST00061W
$240USD    Buy Now 


Rabbit antibody to TRPA1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to TRPA1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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