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Code
OSV00001W
$240USD    Buy Now 

ID Tag
Rb1170-260909-WS

Immunogen
A synthetic peptide from human VACHT conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3, MGC12716

Target
FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.
VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle.
SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ichikawa T, et al. J Chem Neuroanat. 13(1):23-39(1997).
2. Erickson J.D, et al. J. Biol. Chem. 269:21929-21932(1994).

Limitation
For research use only

Code
OSV00001W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 2000 is recommended for WB and to 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for VACHT.

Spcs X-react.
Human, mouse, rat, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Code
GSV00003P
$120USD   

ID Tag
74234-39

Unit size
50 g

Accession

Also known
Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3

Target
This is the immunising peptide for the products Rabbit antibody to VACHT (OSV00002G), Rabbit antibody to VACHT (OSV00001W), Goat antibody to VACHT (OSV00082W)

Purity
>85%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OSV00001W,OSV00002G,OSV00082W

Code
OSV00001W
$240USD    Buy Now 


Rabbit antibody to VACHT IHC-P on paraffin sections of rat spinal cord.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VACHT WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to VACHT
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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