⚬ Services
Code
OSS00033W
$240USD    Buy Now 

ID Tag
Rb321-200308-WS

Immunogen
A synthetic peptide from rat SNAP-25 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and human.

Accession

Also known
SNAP-25, Synaptosomal-associated 25 kDa protein, SNAP25, RIC4, SEC9, SNAP, RIC-4, Super protein, SUP

Target
FUNCTION: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation.
TISSUE SPECIFICITY: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.


Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Madison D.L, et al. J. Neurochem. 72:988-998(1999).

Limitation
For research use only

Related
products
 
Code Product Name
OSS00042G Rabbit antibody to SNAP 25
 

Code
OSS00033W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SNAP-25.

Spcs X-react.
Rat, mouse, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00033W
$240USD    Buy Now 


Rabbit antibody to SNAP 25 WB on rat brain lysate using Rabbit antibody to SNAP 25 (OSS00033W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

⚬ Products
Rabbit antibody to SNAP 25
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides