⚬ Services
Code
OSA00019W
$240USD    Buy Now 

ID Tag
Rb286-270108-WS

Immunogen
A synthetic peptide from c-terminal region of human ATG7 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein

Target
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).

Limitation
For research use only

Code
OSA00019W
$240USD    Buy Now 

Unit size
150 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. Use at a dilution of 1 : 1000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for ATG7.

Spcs X-react.
Human, mouse and rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00019W
$240USD    Buy Now 


Rabbit antibody to ATG7 WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to ATG7
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser � OS2100
Red Carpet: A Histology Carpet for Slides