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Code
OSN00001W
$240USD    Buy Now 

ID Tag
Rb296-230208-WS

Immunogen
Native beta-nerve growth factor (Ngfb, beta-NGF) purified from mouse submandibular gland

Accession

Also known
Ngfb, beta-NGF

Target
NGF is a secreted molecule which plays an important roll in the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Manni L, et al. Neurosci. Lett. 426 (1), 39-44(2007).

Limitation
For research use only

Code
OSN00001W
$240USD    Buy Now 

Unit size
150 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB, inhibition of biological activity. Tested in vitro, this antibody abolishes the neuronal survival and the outgrowth actions of murine NGF in chicken DRG. A dilution of 1 : 20000 to 1 : 30000 is recommended for WB and 1:1000 to 1:2000 for IHC-P; for inhibition of biological activities a dilution of up to 1 : 100 is recommended and for in vivo 1-20 l per gram body weight. The optimal dilution should be determined by the end user.

Specificity
Specific for NGF

Spcs X-react.
Mouse, rat and human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 150 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSN00001W
$240USD    Buy Now 


Rabbit antibody to native NGF WB on rat salivary gland lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:20000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to native NGF IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to native NGF IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to native NGF IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml of Davidsons modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
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Rabbit antibody to native NGF
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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