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Code
OSR00111W
$240USD    Buy Now 

ID Tag
Rb149-281007-WS

Immunogen
A synthetic peptide from aa region 250-300 of rat MRGPRA conjugated to blue carrier protein was used as the antigen.

Accession

Also known
Nuclear receptor MrgA10 RF-amide G protein-coupled receptor, Mrga10, MrgA, Mrgpra, MGC108580

Target
FUNCTION: Orphan receptor activated by a subset of RFamide-family neuropeptides such as FLRF-amide and FMRF-amide. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. May regulate the function of nociceptive neurons by modulation of pain perception.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a subset of IB4-positive small diameter nociceptive dorsal root neurons.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Bender E, et al. Proc. Natl. Acad. Sci. U.S.A. 99:8573-8578(2002).

Limitation
For research use only

Code
OSR00111W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 4000 for WB on rat lysates is recommended and for mouse tissue lysates 1 : 2000 i; 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for MRGPRA.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSR00111W
$240USD    Buy Now 


Rabbit antibody to MRGPRA (250-300) WB on mouse/rat tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution for rat lysates: dilution 1:4000 and for mouse lysates: 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to MRGPRA (250-300)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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