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Code
OSS00430W
$240USD    Buy Now 

ID Tag
Rb2850-090916-WS

Immunogen
A synthetic peptide from mouse SNTB1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

Accession

Also known
Beta-1-syntrophin, 59 kDa dystrophin-associated protein A1 basic component 1, DAPA1B, Syntrophin-2

Target
FUNCTION: Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
SUBCELLULAR LOCATION: Cell membrane › sarcolemma; Peripheral membrane protein; Cytoplasmic side; Cell junction; Cytoplasm › cytoskeleton.
Tissue specificity: Ubiquitous

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Peters M.F, et al. Cell Biol. 138:81-93(1997)

Limitation
For research use only

Code
OSS00430W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 500 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Aggrecan.

Spcs X-react.
Mouse, rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00430W
$240USD    Buy Now 


Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 IHC-P on paraffin sections of mouse heart. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SNTB1 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to SNTB1 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to SNTB1 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to SNTB1 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to SNTB1 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to SNTB1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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