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Code
OSM00117W
$240USD    Buy Now 

ID Tag
Rb2154-011013-WS

Immunogen
A synthetic peptide from aa region 410-460 of human MPP1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide shares 98% identity with mouse sequence.

Accession

Also known
55 kDa erythrocyte membrane protein, p55, palmitoylated 1 Membrane protein, EMP55

Target
Function: Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activit.
Subcellular location: Membrane; Lipid-anchor. Cell projection › stereocilium. Note: Colocalizes with WHRN at stereocilium tip during hair cell development. Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). Colocalizes with NF2 in non-myelin-forming Schwann cells.
Tissue specificity: Ubiquitous

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1.Gosens I, et al. Hum. Mol. Genet. 16:1993-2003(2007)

Limitation
For research use only

Code
OSM00117W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for MPP1.

Spcs X-react.
Human, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSM00117W
$240USD    Buy Now 


Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human brain.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human brain.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human brain.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human kidney.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human kidney.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to MPP1 (410-460) IHC-P on paraffin sections of human small intestine.
HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to MPP1 (410-460)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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