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Code
OSA00214W
$240USD    Buy Now 

ID Tag
Rb1959-091212-WS

Immunogen
A synthetic peptide from human ABCG1 conjugated to blue carrier protein was used as the antigen.

Accession

Also known
ATP-binding cassette sub-family G member 2, Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, CD338, ABCP, BCRP, BCRP1, MXR

Target
FUNCTION: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Subcellular location: Cell membrane; Multi-pass membrane protein
Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Zhou S, et al. Nat. Med. 7:1028-1034(2001)

Limitation
For research use only

Code
OSA00214W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for ABCG2.

Spcs X-react.
Human, marmoset, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00214W
$240USD    Buy Now 


Rabbit antibody to ABCG2 WB on tissues and cell line lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to ABCG2 WB on tissue lysates prepared in different lysing buffers. Note the profound effect of the composition of the lysing buffers on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody dilutions: 1:1000 in Spleen, Skin, Brain; 1:2000 in Testis incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to ABCG2
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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