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Code
OSP00150W
$240USD    Buy Now 

ID Tag
Rb1658-211110-WS

Immunogen
A synthetic peptide from aa region 1120-1162 of mouse KCNH2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Accession

Also known
Potassium voltage-gated channel subfamily H member 2, Eag homolog, Ether-a-go-go-related gene potassium channel 1, ERG-1, Eag-related protein 1, Ether-a-go-go-related protein 1, Voltage-gated potassium channel subunit Kv11.1, ERG, ERG1, HERG

Target
Function: Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr).
Subcellular location: Membrane; Multi-pass membrane protein
Tissue specificity: Highly expressed in brain and testis, slightly less so in heart, adrenal, retina and thymus. Detected at lower levels in lung, soleus, tibialis, and at very low levels in cornea and lens. A shorter transcript is detected in skeletal muscle. Found in pituitary.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. London B, et al. Circ. Res. 81:870-878(1997)
2. Crociani O, et al. J. Biol. Chem. 278:2947-2955(2003)

Limitation
For research use only

Code
OSP00150W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for KCNS2.

Spcs X-react.
Mouse, rat, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSP00150W
$240USD    Buy Now 


Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat brain stem.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat brain stem. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat brain stem. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to KCNH2 (1120-1162) WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
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Rabbit antibody to KCNH2 (1120-1162)
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Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
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