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Code
OSS00285W
$240USD    Buy Now 

ID Tag
Rb1528-130810-WS

Immunogen
A synthetic peptide from human Band 3 anion transport protein (SLC4A1, CD233) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Anion exchange protein 1, AE 1, Anion exchanger 1, Solute carrier family 4 member 1, CD233, SLC4A1, AE1, DI, EPB3

Target
Function: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin.
Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal.
Defects in SLC4A1 are the cause of autosomal dominant distal renal tubular acidosis (AD-dRTA). This disease is characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis.
Defects in SLC4A1 are the cause of autosomal recessive distal renal tubular acidosis (AR-dRTA).
Tissue specificity: Erythrocytes.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Lux S.E, et al. Proc. Natl. Acad. Sci. U.S.A. 86:9089-9093(1989)

Limitation
For research use only

Related
products
 
Code Product Name
OSB00090W Rabbit antibody to mouse SLC4A1
 

Code
OSS00285W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 30000 to 1:100000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SLC4A1.

Spcs X-react.
Human, mouse, rat. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00285W
$240USD    Buy Now 


Rabbit antibody to SLC4A1 WB on mouse blood coagulate lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:30000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to SLC4A1 IHC-P on paraffin sections of human small intestine. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SLC4A1 IHC-P on paraffin sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SLC4A1 IHC-P on paraffin sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SLC4A1 IHC-P on paraffin sections of human tonsil. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to SLC4A1 IHC-P on paraffin sections of human tonsil. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 m. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to SLC4A1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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