⚬ Services
Code
OSL00012W
$240USD    Buy Now 

ID Tag
Rb1359-140310-WS

Immunogen
A synthetic peptide from c-terminal region of human Lingo1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species.

Accession

Also known
LIGO1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobilin domain-containing protein 1, Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, LERN1, LRRN6A

Target
Function: Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination.
Subcellular location: Cell membrane; Single-pass type I membrane protein
Tissue specificity: Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Carim-Todd L, et al. Eur. J. Neurosci. 18:3167-3182(2003)
2. Mosyak L, et al. J. Biol. Chem. 281:36378-36390(2006)

Limitation
For research use only

Code
OSL00012W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 3000 is recommended for WB and 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Lingo1.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSL00012W
$240USD    Buy Now 


Rabbit antibody to Lingo1 WB on brain lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:3000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to Lingo1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser � OS2100
Red Carpet: A Histology Carpet for Slides