A synthetic peptide from c-terminal region of human Lingo1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species.
LIGO1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobilin domain-containing protein 1, Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, LERN1, LRRN6A
Function: Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination.
Subcellular location: Cell membrane; Single-pass type I membrane protein
Tissue specificity: Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Carim-Todd L, et al. Eur. J. Neurosci. 18:3167-3182(2003)
2. Mosyak L, et al. J. Biol. Chem. 281:36378-36390(2006)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 3000 is recommended for WB and 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specific for Lingo1.
Human, rat, mouse. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.