⚬ Services
Code
OSP00105W
$240USD    Buy Now 

ID Tag
Rb3125-241117-WS

Immunogen
A synthetic peptide from rat KCNMA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.

Accession

Also known
Potassium channel subfamily M subunit alpha 1, Calcium-activated potassium channel subunit alpha-1, Calcium-activated potassium channel, subfamily M subunit alpha-1, Maxi K channel, MaxiK, BK channel, K(VCA)alpha, BKCA alpha, KCa1.1, Slowpoke homolog, Slo homolog, Slo-alpha, Slo1, Slo

Target
Function: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Widely expressed. Except in myocytes, it is almost ubiquitously expressed.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Liu Y, et al. Hypertension 30:1403-1409(1997)

Limitation
For research use only

Code
OSP00105W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for KCNMA1.

Spcs X-react.
Rat, mouse, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSP00105W
$240USD    Buy Now 


Rabbit antibody to KCNMA1 WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to KCNMA1
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides