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Code
OST00324W
$240USD    Buy Now 

ID Tag
Gt228-030110-WS

Immunogen
A synthetic peptide from aa region 30-100 of human Tyrosine Hydroxylase conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
TY3H, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, Tyrosine 3 hydroxylase, TH

Target
Function: Plays an important role in the physiology of adrenergic neurons.
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD); also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Mainly expressed in the brain and adrenal glands.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Kaneda N, et al. Biochem. Biophys. Res. Commun. 146:971-975(1987)
2. Grima B, et al. Nature 326:707-711(1987)

Limitation
For research use only

Related
products

Code
OST00324W
$240USD    Buy Now 

Unit size
150 l

Conjugate
Unconjugated antibody

Host
Goat

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 200 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for TH.

Spcs X-react.
Human, rat, mouse, marmoset. Other species not yet tested.

Format
Lyophilised. This product contains 0.02% benzalkonium chloride as a preservative.

Reconstitution
Reconstitute in 150 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OST00324W
$240USD    Buy Now 


Goat antibody to Tyrosine Hydroxylase (30-100) WB on brain lysates. Blocking with 0.5% LFDM for 1 hour at RT; Primary antibody used at 1:500 dilution incubated overnight at 4C.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of mouse brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of mouse brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of mouse olfactory. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human large intestine. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Goat antibody to Tyrosine Hydroxylase (30-100) IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 m Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
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Goat antibody to Tyrosine Hydroxylase (30-100)
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Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
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