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Code
OSS00206W
$240USD    Buy Now 

ID Tag
Rb1184-260909-WS

Immunogen
A synthetic peptide from aa region 40-90 of human SOD1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
SOD1, Superoxide dismutase 1, SODC

Target
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Catalytic activity: 2 superoxide + 2 H(+) = O(2) + H(2)O(2).
Subcellular location: Cytoplasm.
Involvement in disease: Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
The protein (both wild-type and ALS1 variants) has a tendency to form fibrillar aggregates in the absence of the intramolecular disulfide bond or of bound zinc ions. These aggregates may have cytotoxic effects. Zinc binding promotes dimerization and stabilizes the native form.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Sherman L, et al. Proc. Natl. Acad. Sci. U.S.A. 80:5465-5469(1983)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)

Limitation
For research use only

Code
OSS00206W
$240USD    Buy Now 

Unit size
150 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 3000 is recommended for WB and to 1: 1000 fro IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for SOD1.

Spcs X-react.
Human. Other species not yet tested but expected to work in rat, mouse and guinea pig.

Format
Lyophilised

Reconstitution
Reconstitute in 150 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSS00206W
$240USD    Buy Now 


Rabbit antibody to SOD1 (40-90) WB on different lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products
Rabbit antibody to SOD1 (40-90)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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