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Code
OSV00069W
$240USD    Buy Now 

ID Tag
Rb1163-260909-WS

Immunogen
A synthetic peptide from aa region 2-50 of human VGluT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Vesicular glutamate transporter 1, VGlU1, Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Bnpi

Target
FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ni B, et al. Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994).

Limitation
For research use only

Related
products
 
Code Product Name
OSV00086W Goat antibody to VGluT1 (2-50)
 

Code
OSV00069W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
WB, IHC-P, IHC-Fr, ICC, Flow Cyt, ICC/IF. A dilution of 1: 6000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for VGLUT1 (both isoforms 1 and 2).

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSV00069W
$240USD    Buy Now 


Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VGluT1 (2-50) WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.1
Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 m.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 m.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 m.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 m.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
Rabbit antibody to VGluT1 (2-50) IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 m.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.
⚬ Products
Rabbit antibody to VGluT1 (2-50)
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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